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pmscv ires dsred fp vector  (Addgene inc)


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    Structured Review

    Addgene inc pmscv ires dsred fp vector
    Pmscv Ires Dsred Fp Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pmscv+ires+dsred+fp+vector/pMSCV-IRES-DsRed+FP+(pMIDsRed+II)+(Plasmid+%2352110)/pm41195513-217-14-17
    Average 93 stars, based on 5 article reviews
    pmscv ires dsred fp vector - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Uracil-DNA glycosylase of murine gammaherpesvirus 68 binds cognate viral replication factors independently of its catalytic residues.
    Article Snippet: .. Briefly, the DsRed gene was PCR amplifed from the pMSCV-IRES-DsRed Fp vector (Addgene, Watertown, MA, USA) and cloned via HindIII and KpnI into the pBlueScript KS+ vec tor to generate pBS_DsRed. .. A synthetic KanR cassette (gBLOCK) (42) (Integrated DNA Technologies, Coralville, IA, USA) was constructed so that it contained the IscEI hom ing enzyme recognition site as well as a 50-bp region homologous to the 50 bp immediately downstream of the PstI restriction site of pBS_DsRed.

    Article Title: Uracil-DNA glycosylase of murine gammaherpesvirus 68 binds cognate viral replication factors independently of its catalytic residues
    Article Snippet: .. Briefly, the DsRed gene was PCR amplifed from the pMSCV-IRES-DsRed Fp vector (Addgene, Watertown, MA, USA) and cloned via HindIII and KpnI into the pBlueScript KS+ vector to generate pBS_DsRed. .. A synthetic KanR cassette (gBLOCK) ( ) (Integrated DNA Technologies, Coralville, IA, USA) was constructed so that it contained the IscEI homing enzyme recognition site as well as a 50-bp region homologous to the 50 bp immediately downstream of the PstI restriction site of pBS_DsRed.

    Article Title: Uracil-DNA Glycosylase of Murine Gammaherpesvirus 68 Binds Cognate Viral Replication Factors Independently of its Catalytic Residues
    Article Snippet: .. Briefly, the DsRed gene was PCR amplifed from the pMSCV-IRES-DsRed Fp vector (Addgene, Watertown, MA, USA) and cloned via HindIII and KpnI into the pBlueScript KS+ vector to generate pBS_DsRed. .. A synthetic KanR cassette (gBLOCK) ( ) (Integrated DNA Technologies, Coralville, IA) was constructed so that it contained the IscEI homing enzyme recognition site as well as a 50bp region homologous to the 50bp immediately downstream of the PstI restriction site of pBS_DsRed.

    Article Title: Uracil-DNA Glycosylase of Murine Gammaherpesvirus 68 Binds Cognate Viral Replication Factors Independently of its Catalytic Residues
    Article Snippet: .. Briefly, the DsRed gene was PCR amplifed from the pMSCV-IRES-DsRed Fp vector (Addgene, Watertown, MA, USA) and cloned via HindIII and KpnI into the pBlueScript KS+ vector to generate pBS_DsRed. .. A synthetic KanR cassette (gBLOCK) ( ) (Integrated DNA Technologies, Coralville, IA) was constructed so that it contained the IscEI homing enzyme recognition site as well as a 50bp region homologous to the 50bp immediately downstream of the PstI restriction site of pBS_DsRed.

    Clone Assay:

    Article Title: Uracil-DNA glycosylase of murine gammaherpesvirus 68 binds cognate viral replication factors independently of its catalytic residues.
    Article Snippet: .. Briefly, the DsRed gene was PCR amplifed from the pMSCV-IRES-DsRed Fp vector (Addgene, Watertown, MA, USA) and cloned via HindIII and KpnI into the pBlueScript KS+ vec tor to generate pBS_DsRed. .. A synthetic KanR cassette (gBLOCK) (42) (Integrated DNA Technologies, Coralville, IA, USA) was constructed so that it contained the IscEI hom ing enzyme recognition site as well as a 50-bp region homologous to the 50 bp immediately downstream of the PstI restriction site of pBS_DsRed.

    Article Title: Uracil-DNA glycosylase of murine gammaherpesvirus 68 binds cognate viral replication factors independently of its catalytic residues
    Article Snippet: .. Briefly, the DsRed gene was PCR amplifed from the pMSCV-IRES-DsRed Fp vector (Addgene, Watertown, MA, USA) and cloned via HindIII and KpnI into the pBlueScript KS+ vector to generate pBS_DsRed. .. A synthetic KanR cassette (gBLOCK) ( ) (Integrated DNA Technologies, Coralville, IA, USA) was constructed so that it contained the IscEI homing enzyme recognition site as well as a 50-bp region homologous to the 50 bp immediately downstream of the PstI restriction site of pBS_DsRed.

    Article Title: Uracil-DNA Glycosylase of Murine Gammaherpesvirus 68 Binds Cognate Viral Replication Factors Independently of its Catalytic Residues
    Article Snippet: .. Briefly, the DsRed gene was PCR amplifed from the pMSCV-IRES-DsRed Fp vector (Addgene, Watertown, MA, USA) and cloned via HindIII and KpnI into the pBlueScript KS+ vector to generate pBS_DsRed. .. A synthetic KanR cassette (gBLOCK) ( ) (Integrated DNA Technologies, Coralville, IA) was constructed so that it contained the IscEI homing enzyme recognition site as well as a 50bp region homologous to the 50bp immediately downstream of the PstI restriction site of pBS_DsRed.

    Article Title: Uracil-DNA Glycosylase of Murine Gammaherpesvirus 68 Binds Cognate Viral Replication Factors Independently of its Catalytic Residues
    Article Snippet: .. Briefly, the DsRed gene was PCR amplifed from the pMSCV-IRES-DsRed Fp vector (Addgene, Watertown, MA, USA) and cloned via HindIII and KpnI into the pBlueScript KS+ vector to generate pBS_DsRed. .. A synthetic KanR cassette (gBLOCK) ( ) (Integrated DNA Technologies, Coralville, IA) was constructed so that it contained the IscEI homing enzyme recognition site as well as a 50bp region homologous to the 50bp immediately downstream of the PstI restriction site of pBS_DsRed.

    Plasmid Preparation:

    Article Title: Uracil-DNA glycosylase of murine gammaherpesvirus 68 binds cognate viral replication factors independently of its catalytic residues
    Article Snippet: .. Briefly, the DsRed gene was PCR amplifed from the pMSCV-IRES-DsRed Fp vector (Addgene, Watertown, MA, USA) and cloned via HindIII and KpnI into the pBlueScript KS+ vector to generate pBS_DsRed. .. A synthetic KanR cassette (gBLOCK) ( ) (Integrated DNA Technologies, Coralville, IA, USA) was constructed so that it contained the IscEI homing enzyme recognition site as well as a 50-bp region homologous to the 50 bp immediately downstream of the PstI restriction site of pBS_DsRed.

    Article Title: Uracil-DNA Glycosylase of Murine Gammaherpesvirus 68 Binds Cognate Viral Replication Factors Independently of its Catalytic Residues
    Article Snippet: .. Briefly, the DsRed gene was PCR amplifed from the pMSCV-IRES-DsRed Fp vector (Addgene, Watertown, MA, USA) and cloned via HindIII and KpnI into the pBlueScript KS+ vector to generate pBS_DsRed. .. A synthetic KanR cassette (gBLOCK) ( ) (Integrated DNA Technologies, Coralville, IA) was constructed so that it contained the IscEI homing enzyme recognition site as well as a 50bp region homologous to the 50bp immediately downstream of the PstI restriction site of pBS_DsRed.

    Article Title: Loss of KDEL function from a calreticulin frameshift mutation drives expression of an immature, mutant calreticulin-dependent form of the thrombopoietin receptor MPL.
    Article Snippet: .. Supplemental Information Supplemental methods Plasmids To express untagged MPL, cDNA was subcloned into the pMSCV-IRES-DsRed FP vector (Addgene #52110). .. Cell culture and proliferation assay Interleukin (IL)-3-dependent Ba/F3 cells were cultured at 37°C in 5% CO2 in Roswell Park Memorial Institute 1640 medium (Nacalai Tesque, #30264-56) containing 10% fetal bovine serum (Thermo Fisher, #10270-106), 1 ng/ml murine IL-3 (PEPROTECH, #213-13- 50UG), 100 U/mL penicillin (Nacalai Tesque, #26239-42), and 100 μg/mL streptomycin (Nacalai Tesque, #32204-92).

    Article Title: Uracil-DNA Glycosylase of Murine Gammaherpesvirus 68 Binds Cognate Viral Replication Factors Independently of its Catalytic Residues
    Article Snippet: .. Briefly, the DsRed gene was PCR amplifed from the pMSCV-IRES-DsRed Fp vector (Addgene, Watertown, MA, USA) and cloned via HindIII and KpnI into the pBlueScript KS+ vector to generate pBS_DsRed. .. A synthetic KanR cassette (gBLOCK) ( ) (Integrated DNA Technologies, Coralville, IA) was constructed so that it contained the IscEI homing enzyme recognition site as well as a 50bp region homologous to the 50bp immediately downstream of the PstI restriction site of pBS_DsRed.



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